Journal: Frontiers in Endocrinology

Article Title: Diagnostic Role of Prostate-Specific Membrane Antigen in Adrenocortical Carcinoma

doi: 10.3389/fendo.2019.00226

Figure Lengend Snippet: The staining of PSMA and CD34 in ACA and ACC (X200). As we can see from the figure, the staining of PSMA in CPA, APA, NFT and metastases were negative, while in ACC it was positive with high percentage.

Article Snippet: The slides were stained for CD34 (1:200; ZM-0046; Origene) and PSMA (diluted 1:100; ab19071; Abcam).

Techniques: Staining

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunohistochemical staining results of young and adult Tibetan sheep myocardium(scale bar, 10 μm). Immunohistochemical staining results of young and adult Tibetan sheep myocardium(scale bar, 10 µm).A-a: The expression of CD34 in the myocardium of young Tibetan sheep . A-b: The expression of CD117 in the myocardium of young Tibetan sheep. A-c: The expression of PDGFR-α in the myocardium of young Tibetan sheep. A-d: The expression of α-SMA in the myocardium of young Tibetan sheep. B-a: The expression of CD34 in the myocardium of adult Tibetan sheep. B-b: The expression of CD117 in the myocardium of adult Tibetan sheep. B-c: The expression of PDGFR-α in the myocardium of adult Tibetan sheep. B-d: The expression of α-SMA in the myocardium of adult Tibetan sheep. Black arrows indicate TCs

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunohistochemical staining results of young and adult Tibetan sheep heart aortic bulb(scale bar, 10 μm). A-a: The expression of CD34 in the aortic bulb of young Tibetan sheep . A-b: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-c: The expression of PDGFR-α in the aortic bulb of young Tibetan sheep. A-d: The expression of α-SMA in the aortic bulb of young Tibetan sheep. B-a: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-b: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-c: The expression of PDGFR-α in the aortic bulb of adult Tibetan sheep. B-d: The expression of α-SMA in the aortic bulb of adult Tibetan sheep. Black arrows indicate TCs

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunofluorescence double staining results of young and adult Tibetan sheep myocardium (scale bar, 50 μm). A-a: The expression of CD34 in the myocardium of young Tibetan sheep. A-b: The expression of CD117 in the myocardium of young Tibetan sheep. A-c: The expression of DAPI in the myocardium of young Tibetan sheep. A-d: Co-expression of CD34 and CD117 in the myocardium of young Tibetan sheep. A-e: The expression of CD34 in the myocardium of young Tibetan sheep. A-f: The expression of α-SMA in the myocardium of young Tibetan sheep. A-g: The expression of DAPI in the myocardium of young Tibetan sheep. A-h: The co-expression of CD34 and α-SMA in the myocardium of young Tibetan sheep. A-i: The expression of PDGFR-α in the myocardium of young Tibetan sheep. A-j: The expression of CD117 in the myocardium of young Tibetan sheep. A-k: The expression of DAPI in the myocardium of young Tibetan sheep. A-l: Co-expression of PDGFR-α and CD117 in the myocardium of young Tibetan sheep. B-a: The expression of CD34 in the myocardium of adult Tibetan sheep. B-b: The expression of CD117 in the myocardium of adult Tibetan sheep. B-c: The expression of DAPI in the myocardium of adult Tibetan sheep. B-d: Co-expression of CD34 and CD117 in the myocardium of adult Tibetan sheep. B-e: The expression of CD34 in the myocardium of adult Tibetan sheep. B-f: The expression of α-SMA in the myocardium of adult Tibetan sheep. B-g: The expression of DAPI in the myocardium of adult Tibetan sheep. B-h: The co-expression of CD34 and α-SMA in the myocardium of adult Tibetan sheep. B-i: The expression of PDGFR-α in the myocardium of adult Tibetan sheep. B-j: The expression of CD117 in the myocardium of adult Tibetan sheep. B-k: The expression of DAPI in the myocardium of adult Tibetan sheep. B-l: Co-expression of PDGFR-α and CD117 in the myocardium of adult Tibetan sheep

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunofluorescence, Double Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunofluorescence double staining results of young and adult Tibetan sheep heart aortic bulb (scale bar, 50 μm). A-a: The expression of CD34 in the aortic bulb of young Tibetan sheep. A-b: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-c: The expression of DAPI in the aortic bulb of young Tibetan sheep . A-d: Co-expression of CD34 and CD117 in the aortic bulb of young Tibetan sheep . A-e: The expression of CD34 in the aortic bulb of young Tibetan sheep. A-f: The expression of α-SMA in the aortic bulb of young Tibetan sheep. A-g: The expression of DAPI in the aortic bulb of young Tibetan sheep. A-h: The co-expression of CD34 and α-SMA in the aortic bulb of young Tibetan sheep . A-i: The expression of PDGFR-α in the aortic bulb of young Tibetan sheep. A-j: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-k: The expression of DAPI in the aortic bulb of young Tibetan sheep. A-l: Co-expression of PDGFR-α and CD117 in the aortic bulb of young Tibetan sheep. B-a: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-b: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-c: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-d: Co-expression of CD34 and CD117 in the aortic bulb of adult Tibetan sheep. B-e: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-f: The expression of α-SMA in the aortic bulb of adult Tibetan sheep. B-g: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-h: The co-expression of CD34 and α-SMA in the aortic bulb of adult Tibetan sheep. B-i: The expression of PDGFR-α in the aortic bulb of adult Tibetan sheep. B-j: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-k: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-l: Co-expression of PDGFR-α and CD117 in the aortic bulb of adult Tibetan sheep.

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunofluorescence, Double Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Protein expression of effective telocyte markers (CD117, CD34, α-SMA and PDGFR-α) in the myocardium and aortic bulb of young and adult Tibetan sheep. ( a ) Western blotting, ( b ) CD117 Relative protein expression levels, ( c ) CD34 Relative protein expression levels, ( d ) α-SMA Relative protein expression levels, ( e ) PDGFR-α Relative protein expression levels. Each sample was tested three times. Results are expressed as the mean±standard deviation, with β-actin protein expression used as an internal control. *** P <0.001, ns (not significant), P >0.05

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Frontiers in Neuroscience

Article Title: Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion

doi: 10.3389/fnins.2021.759325

Figure Lengend Snippet: CD34 immunohistochemical staining. (A) Sham group; (B) injury group; (C) T50 group; (D) T100 group; (E) statistical results of microvascular density showed that MVD of injury group, T50 group and T100 group were significantly lower than Sham group, and the MVD of T50 group and T100 group were significantly higher than injury group. * P < 0.05, comparison between each group and sham group; # P < 0.05, comparison between T50 group or T100 group and injury group.

Article Snippet: The resulting sections were soaked with 3% hydrogen peroxide to block endogenous peroxidase activity and then incubated overnight with rabbit polyclonal anti-CD34 antibody (1:1,000; Bioworld Technology, St. Louis Park, MN, United States).

Techniques: Immunohistochemical staining, Staining